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KMID : 0854720080280030199
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Korean Journal of Asthma, Allergy and Clinical Immunology
2008 Volume.28 No. 3 p.199 ~ p.204
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Difference of Dermatophagoides farinae Specific IgE Binding Patterns between Asthmatics and Atopic Dermatitis Patients
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Kim Gyu-Ri
Kim Min-Hwan
Chou Soo-Young
Lee Yong-Won
Park Jung-Won
Hong Chein-Soo
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Abstract
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Background: Dermatophagoides farinae (D. farinae) has various isoallergens. However, allergenic differences between bronchial asthmatics (BA) and atopic dermatitis (AD) patients have not yet been fully evaluated.
Objective: To investigate the differences in D. farinae specific IgE between BA and AD patients sensitized to D.
farinae.
Method: Five BA and five AD patients sensitized to D. farinae were measured in this study. D. farinae-specific IgE
was evaluated by 1-dimensional (1-D) and 2-dimensional (2-D) SDS-PAGE, and IgE immunoblotting. N-terminal sequencing was performed for the identification of the allergens.
Result: Two-D IgE immunoblot revealed the 7 allergens with 14 kDa at PI 5.5 to 6.5. These allergens were found
in 4, BA and 2 AD patients. Another allergen with 25 kDa at pI 7.8 was found only in 4 BA patients. This allergen was regarded as triosephosphate isomerase by N-terminal sequencing.
Conclusion: Seven allergens with 14 kDa at pI 5.5 to 6.5 and 1 allergen with 25 kDa at pI 7.8 were identified more
frequently in BA than in AD patients. The 25 kDa allergen was regarded as triosephosphate isomerase, and it might be
associated with the development of asthma.
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KEYWORD
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Dermatophagoides farinae, Allergens, Asthma, Atopic dermatitis, Triosephosphate isomerase
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